Journal: Cell reports

Article Title: Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells

doi: 10.1016/j.celrep.2019.05.077

Figure Lengend Snippet: IF images from cryosection stains from day 40 WT lung organoids cultured in the presence or absence or rhIL-11, as well as HPS1−/−, HPS2−/−, HPS4−/−, and HPS8−/− organoids. Red and green: corresponding IF markers indicated for each stain. Blue: DAPI stain. Scale bar: 100 μm.

Article Snippet: AP3B1 (HPS2) , Proteintech Group , Cat#13384–1-AP, RRID:AB_2056499.

Techniques: Cell Culture, Staining

Journal: Cell reports

Article Title: Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells

doi: 10.1016/j.celrep.2019.05.077

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: AP3B1 (HPS2) , Proteintech Group , Cat#13384–1-AP, RRID:AB_2056499.

Techniques: Recombinant, RNAscope, Multiplex Assay, Positive Control, Enzyme-linked Immunosorbent Assay, Sequencing, Plasmid Preparation, Software

Journal: Technology in Cancer Research & Treatment

Article Title: In Vitro and In Vivo Analyses Reveal Tumor-Derived Exosome miR-558 Promotes Angiogenesis in Tongue Squamous Cell Carcinoma by Targeting Heparinase

doi: 10.1177/15330338241261615

Figure Lengend Snippet: miR-558 regulates HPSE to promote proliferation, migration, and tube formation in HUVECs. (A) Construction of wild-type and mutant luciferase-containing plasmid sequences of predicted binding sites between HPSE and miR-558. (B) Co-transfect HPSE wild-type and mutant luciferin-containing plasmids for the miR-558 binding site in HUVEC cells, as well as miR-558 mimics or NC mimics, and detect the luciferase activity to reflect the binding situation. (C) The expression level of HPSE in HUVECs after overexpression of miR-558 was detected by qRT-PCR. (D) Protein expression of HPSE, VEGF-A and MMP9 in HUVEC after overexpression of miR-558. (E) After transfecting HUVEC with three small interfering RNAs and one control sequence, the expression level of HPSE was detected by qRT-PCR experiment. (F) The effect of knocking out HPSE on the proliferation ability of HUVEC overexpressing miR-558. (G–H) Representative images of migration and tube formation of miR-558-overexpressed HUVECs knocked out of HPSE, the number of migrated cells and the number of tubes are shown in the right chart. * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001.

Article Snippet: HUVEC was plated in a six-well plate, 2 mL opti-MEM low-serum medium was added, 4 μg plasmid was dissolved in 250 μL opti-MEM low-serum medium, 10 μL Lipofectamine 2000 was added to opti-MEM low-serum medium, plasmid mixed with Lipofectamine 2000 was added to HUVEC medium, and the cell supernatant was discarded after 6 h, and further experiments were carried out after culturing for 24 h. Small-interfering RNAs (siRNAs) of HPSE together with their negative control were synthesized with Ribobio (Guangzhou, China): siRNA-1,5′-CTAACAGTTTCCTTAAGAA-3′; siRNA-2,5′-GAAGGAAGCTTCGAGTATA-3′; siRNA-3,5′-CCATAAACCTCCATAATGT-3′.

Techniques: Migration, Mutagenesis, Luciferase, Plasmid Preparation, Binding Assay, Activity Assay, Expressing, Over Expression, Quantitative RT-PCR, Control, Sequencing